This evening session will start with a 20′ theoretical introduction on sample preparation for some of the advanced experimental methods in structural biology, including serial crystallography and cryoEM (see the abstract below).
After the lecture, the session will continue with a demo on automatic crystallization setup and all brought samples will be put to crystallize.
A1_L2 Preparing crystal samples for advanced crystallographic methods
Patrick D. Shaw Stewart, Stefan A. Kolek, Jack Stubbs, Peter Baldock
Douglas Instruments Ltd,
Douglas House, East Garston, Berkshire, RG17 7HD, UK
Advanced crystallographic methods require diffracting crystals of specific sizes – very small for microED, small and uniform for serial data collection, and very large for neutron diffraction. Microseeding into the metastable zone of the crystallization phase diagram allows the number and size of crystals to be controlled. We recommend the same fundamental work-flow for preparing crystal samples for these three data-collection methods: (1) identify metastable conditions in a microbatch-under-oil format where microseeding is effective; (2) construct a simple phase diagram in microbatch; (3) scale up experiments in a microbatch format (this is much easier than scaling up in vapor diffusion). Case studies will be shown together with suggestions for tailoring the approach to the individual data-collection methods.
